COL2A1 Antikörper (Chain alpha 1) (Biotin)

Produktnummer ABIN116553

Produktdetails anti-COL2A1 Antikörper (Biotin)

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Bindungsspezifität: Chain alpha 1

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL2A1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: Crossreactivity < 1 % to other collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Rat, Bovine, Sheep.

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Aufreinigung: Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogen: Collagen type II purified from adult Human knee and Bovine nasal cartilage. For immunization collagens extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein.

Anwendungsinformationen

Applikationshinweise: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended dilutions: ELISA 1: 10,000 - 1: 50,000Western blot 1: 5,000 - 1: 10,000Immunohistochemistry 1: 200 - 1: 1,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.1 M Sodium Borate, 0.071 M Sodium Chloride, 0.001 M EDTA, pH 8.0, 0.01 % Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Details zu COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Abstract: COL2A1 Produkte

Synonyme: ANFH antikoerper, AOM antikoerper, COL11A3 antikoerper, SEDC antikoerper, Col2 antikoerper, Col2a antikoerper, Col2a-1 antikoerper, Del1 antikoerper, Dmm antikoerper, Lpk antikoerper, M100856 antikoerper, Rgsc856 antikoerper, CG2A1A antikoerper, COLLII antikoerper, col2a1a antikoerper, CB11 antikoerper, col2a1 antikoerper, coll2a1 antikoerper, fb38c06 antikoerper, fc10c01 antikoerper, wu:fb38c06 antikoerper, wu:fc10c01 antikoerper, collagen type II alpha 1 chain antikoerper, collagen, type II, alpha 1 antikoerper, collagen, type II, alpha 1 L homeolog antikoerper, collagen, type II, alpha 1 S homeolog antikoerper, collagen, type II, alpha 1a antikoerper, COL2A1 antikoerper, Col2a1 antikoerper, col2a1 antikoerper, col2a1.L antikoerper, col2a1.S antikoerper, col2a1a antikoerper

Hintergrund: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: Alpha-1 type II collagen, COL2A1

Gen-ID: 1280

NCBI Accession: NP_001835

UniProt: P02458

Pathways: Sensory Perception of Sound, Growth Factor Binding