TNFRSF1B Antikörper

Produktnummer ABIN116477

Der Kaninchen Polyklonal anti-TNFRSF1B Antikörper wird verwendet zum Nachweis von TNFRSF1B in Proben von Human. Er wurde validiert für WB, IF und EIA.

Kurzübersicht für TNFRSF1B Antikörper (ABIN116477)

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNFRSF1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-TNFRSF1B Antikörper

Aufreinigung: Affinity Chromatography employing immobilized hsTNF Receptor II matrix.

Immunogen: Highly purified (>98%) Ecoli derived recombinant hsTNF Receptor II (Human soluble Tumor Necrosis Factor Receptor II).

Anwendungsinformationen

Applikationshinweise: ELISA: To detect hsTNF-receptor II by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hsTNF-receptor II. To detect hsTNF-receptor II by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with a biotinylated Anti-Human sTNF-receptor II (PP1222B) as adetection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinanthsTNF-receptor II. Western Blot: This antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hsTNF-receptor II is1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu TNFRSF1B

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Andere Bezeichnung: CD120b / TNFR2

Hintergrund: Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor

Gen-ID: 9606

UniProt: P20333

Pathways: NF-kappaB Signalweg, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway