ELISA. Western Blotting. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: Immunohistochemical detection of Tenascinis valuable for studies of tissue differentiation and tumour growth. The antibody isexcellent for staining of paraffin-embedded tissue sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Gulubova: "Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis." in: Pathology, research and practice, Vol. 198, Issue 12, pp. 803-12, (2003) (PubMed).
Faustino, van Garderen, Schalken, Nederbragt: "Tenascin expression in normal, hyperplastic, dysplastic and neoplastic canine mammary tissues." in: Journal of comparative pathology, Vol. 126, Issue 1, pp. 1-8, (2002) (PubMed).
Ioachim, Charchanti, Briasoulis, Karavasilis, Tsanou, Arvanitis, Agnantis, Pavlidis et al.: "Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and ..." in: European journal of cancer (Oxford, England : 1990), Vol. 38, Issue 18, pp. 2362-70, (2002) (PubMed).
Bártek, Viklický, Fran?k, Angelisová, Dráber, Jarosíková, N?mec, Verlová: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, (1982) (PubMed).
tn antikoerper, 150-225 antikoerper, GMEM antikoerper, GP antikoerper, HXB antikoerper, JI antikoerper, TN antikoerper, TN-C antikoerper, AI528729 antikoerper, C130033P17Rik antikoerper, Hxb antikoerper, Ten antikoerper, cytotactin antikoerper, tenascin-C antikoerper, P230 antikoerper, tenc antikoerper, wu:fk04d02 antikoerper, tenascin C L homeolog antikoerper, tenascin C antikoerper, tnc.L antikoerper, TNC antikoerper, MCYG_00667 antikoerper, tnc antikoerper, Tnc antikoerper
Hintergrund
Tenascin is a high molecular ECM glycoprotein (MW app. 210-300 kDa), which contains hexamere repeat-rich single chains. It contains EGF-, Fibronectin III- and Fibronectin-similar structures. During organogenesis it is exprimed temporarily by many organs. During adulthood it reappears only in regeneration, wound healing and in stroma of various tumours. It probably has an anti-adhesive function, which plays a role during tumour speading. Tenascin is found in the central and peripheral nerve system, in smooth muscle, tendon and hyper-proliferative skin.Synonyms: Cytotactin, GMEM, GP 150-225, Glioma-associated-extracellular matrix antigen, Hexabrachion, Myotendinous antigen, Neuronectin, TN-C, Tenascin-C