In den Warenkorb
Bestellen Sie auch über:
+49 (0)241 95 163 153
+49 (0)241 95 163 155

CD14 Molecule (CD14) Antikörper (Biotin)

Antigen

CD14 Molecule (CD14)

Synonyme CD14
Klonalität Monoklonal (X-8)
Wirt
Reaktivität
Alternativen

Human

Konjugat
Alternativen Biotin
Applikation
Alternativen Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS)
Produktnummer ABIN111908
Menge 0.2 mg  (0.4 mg/ml)
Preis 380,00 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung

Produktmerkmale Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
Weitere Bezeichnung CD14
Swiss-Prot P08571
Isotyp IgG2b
Klon X-8
Beschreibung CD14 is a 53'000 - 55'000 Mr glycoprotein strongly expressed on the surface of monocytesand weakly on the surface of granulocytes. It is anchored to the surface by linkage toglycosyl phosphatidylinositol (GPI). CD14 functions as a receptor for endotoxin and is themajor receptor involved in the lethal response to both endotoxin and Gram-negativebacteria. In addition to membrane CD14, soluble forms of CD14 can be detected in serumand tissue culture supernatants of cells transfected with CD14.
Spezifität Monoclonal antibody X-8 prevents the Lipopolysaccharide Binding Protein (LBP)independent activation of cells via soluble CD14 by blocking the binding of CD14.

Anwendungen

Applikationshinweise Suitable for Immunohistochemistry (IHC) on frozen sections (0.25-0.5 ug/ml,1/800-1/1600) and FACS. Suggested positive control: Human placenta. Antigen Distribution on Isolated cells and Tissue sections: Monocytes, granulocytes, macrophages in myeloid leukaemia. Cell Lines: HL-60 without stimulation negativeHL-60 stimulated with PMA 15% positiveHL-60 stimulated with DMSO 15% positiveU937 negativeX-8 is positive on Langerhans cells, follicular dendritic cells, histiocytes and highendothelial cell venules. Functional assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Konzentration 0.4 mg/ml
Buffer PBS, pH 7.2 with 5 mg/ml bovine serum albumin (BSA) as a stabilizerwithout preservatives.Label: Biotin.Reconstitution: Restore with 0.5 ml distilled water.
Lagerung Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutionsAvoid repeated freezing and thawing. Shelf life: One year from despatch.
Forschungsgebiet Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "CD14 Molecule (CD14)", Typ "Antikörper" finden
Wirte Maus (364), Kaninchen (41), Ratte (35), Ziege (9), Huhn (3)
Reaktivitäten Human (388), Maus (63), Hund (54), Affe (41), Schwein (37), Rind (Kuh) (32), Schaf (28), Cynomolgus (25), Primate (25), Rhesusaffen (25), Kaninchen (24), Primaten (19), Ratte (Rattus) (19), Katze (9), Ziege (9), Pferd (2), Pavian (1), Huhn (1), Schimpanse (1), Llama (1)
Applikationen Durchflusszytometrie (FACS) (368), Western Blot (WB) (69), Immunpräzipitation (IP) (58), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (45), ELISA (36), Immunfluoreszenz (IF) (35), Enzyme Immunoassay (EIA) (32), Funktionale Studien (Func) (32), Immunhistochemie (IHC) (28), Immunhistochemie (Paraffinschnitte) (IHC (p)) (27), Blockierende Antikörper (Inhibition) (5), ELISA (Detektionsantikörper) (5), Immunzytochemie (ICC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Konjugate FITC (55), PE (46), Biotin (38), APC (27), PerCP,Cy5.5 (10), Pacific Blue (8), RPE (8), Alexa Fluor 488 (6), PE,Cy7 (6), APC,Cy7 (5), Alexa Fluor 647 (5), Cy5.5 (5), Alexa Fluor 700 (4), Cy5 (2), Cy7,PE (2), EFluor 450 (2), EFluor 605NC (2), PE,Cy5 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), CF (1), Cy3 (1), Cy7 (1), DY590 (1), DY647 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitope C-Term (2), N-Term (2)