This Monoclonal antibody F1G4 reacts with GnRH receptors in the anterior pituitary. F1G4 showed specificity for the synthetic peptide in ELISA and Dot blot.
Kreuzreaktivität (Details)
Species reactivity (tested):Human.
Aufreinigung
Purified
Immunogen
Mice were immunized with a BSA-conjugated peptide corresponding to amino acids 1-29 of Human GnRH receptor extracellular domain. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability was established by subcloning four times.
Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). The receptor contains of seven hydrophobic transmembrane domains connected by hydrophillic extracellular, and intracellular loops characteristic of G-protein coupled receptors.Gonadotropin Releasing Hormone (GnRH) is down-regulated by hCG and believed to be an autocrine factor that regulates the ovary. The Gonadotropin Releasing Hormone Receptor (GnRHR) is synthesized in the pituitary gland. Synonyms: GRHR, GnRH-R, Gonadotropin-releasing hormone receptor