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SLC7A2 Antikörper (Internal Region)

SLC7A2 Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1049350
  • Target Alle SLC7A2 Antikörper anzeigen
    SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))
    Bindungsspezifität
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 13
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Schwein, Kaninchen
    Wirt
    • 13
    Kaninchen
    Klonalität
    • 13
    Polyklonal
    Konjugat
    • 8
    • 3
    • 1
    • 1
    Dieser SLC7A2 Antikörper ist unkonjugiert
    Applikation
    • 4
    • 4
    • 4
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human SLC7A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TPR (67 % ).
    Homologie
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Rabbit, Pig (100%) Orangutan, Gibbon, Galago, Marmoset, Hamster, Bovine, Bat, Horse (92%) Panda, Dog, Platypus (83%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic 12 amino acid peptide from internal region of human SLC7A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Rabbit, Pig (100%), Orangutan, Gibbon, Galago, Marmoset, Hamster, Bovine, Bat, Horse (92%), Panda, Dog, Platypus (83%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product SLC7A2 Primärantikörper
  • Applikationshinweise
    Approved: IHC, IHC-P (15 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.
    Kommentare

    Target Species of Antibody: Human

    Testdurchführung

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    SLC7A2 (Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 2 (SLC7A2))
    Andere Bezeichnung
    SLC7A2 (SLC7A2 Produkte)
    Synonyme
    slc7a2 antikoerper, CAT2 antikoerper, CAT-2 antikoerper, MGC83504 antikoerper, zgc:103686 antikoerper, ATRC2 antikoerper, HCAT2 antikoerper, AI158848 antikoerper, Atrc2 antikoerper, Cat2 antikoerper, Tea antikoerper, Cat2a antikoerper, Cat2b antikoerper, RCAT2 antikoerper, cCAT-2 antikoerper, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2, gene 2 L homeolog antikoerper, solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 antikoerper, solute carrier family 7 member 2 antikoerper, slc7a2.2.L antikoerper, slc7a2 antikoerper, SLC7A2 antikoerper, Slc7a2 antikoerper
    Hintergrund
    Name/Gene ID: SLC7A2
    Subfamily: Cationic amino acid transporter
    Family: Transporter

    Synonyms: SLC7A2, ATRC2, CAT2, HCAT2, CAT-2
    Gen-ID
    6542
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