CCL4 Antikörper (Chemokine (C-C Motif) Ligand 4) (Biotin)

Details for Product anti-CCL4 Antibody No. ABIN1043811, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACT2
  • AT744.1
  • G-26
  • HC21
  • LAG-1
  • LAG1
  • MIP-1-beta
  • MIP1B
  • MIP1B1
  • SCYA2
  • SCYA4
  • CCL4
  • K203
  • Mip1-b
  • Scya4
  • MIP-1beta
  • MIP-1BETA
  • MIP1b
  • SCY4A
  • Act-2
  • MIP-1B
  • Mip1b
  • C-C motif chemokine ligand 4
  • chemokine (C-C motif) ligand 4
  • CCL4
  • Ccl4
Reaktivität
Human
168
77
41
14
6
6
5
2
1
1
1
Wirt
Kaninchen
177
63
4
4
3
2
1
Klonalität
Polyklonal
Konjugat
Dieser CCL4 Antikörper ist konjugiert mit Biotin
26
14
12
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
201
124
58
37
27
23
18
17
16
13
8
7
5
4
3
3
3
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen Human MIP-1 beta Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
Immunogen Type: RecombinantProtein
Isotyp IgG
Spezifität MIP-1 beta Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Produktmerkmale MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. Immunology Research
Andere Bezeichnung MIP-1 beta (CCL4 Antibody Abstract)
Hintergrund MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. Immunology Research
Synonyms: CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß
Gen-ID 6351
NCBI Accession NP_002975
UniProt P13236
Applikationshinweise Human MIP-1 beta Antibody Biotin Conjugated has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research
Kommentare

Gene Name: CCL4

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Produkt verwendet in: Guan, Wang, Roderiquez, Norcross: "Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 32348-52, 2002 (PubMed).

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