Maus anti-Human Immunoglobulin kappa Chain Complex (Igk) Antikörper

Produktnummer ABIN7881161

Kurzübersicht für Maus anti-Human Immunoglobulin kappa Chain Complex (Igk) Antikörper (ABIN7881161)

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: SPM558

Isotyp: IgG1, kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Anti-Kappa Antibody

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human Ig kappa chain was used as the immunogen for this anti-Kappa antibody.

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-Kappa antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Kappa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Antigendetails

Target: Igk (Immunoglobulin kappa Chain Complex (Igk))

Andere Bezeichnung: Kappa

Hintergrund: This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

UniProt: P01601