Sonic Hedgehog Protein (SHH)
Kurzübersicht für Sonic Hedgehog Protein (SHH) (ABIN935799)
Target
Alle Sonic Hedgehog (SHH) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Reinheit
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Sequenz
- IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
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Produktmerkmale
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Purified recombinant Human Sonic Hedgehog protein
Expression System: E.coli
Bioactivity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 ?g/mL. -
Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- < 0.1 ng per µg (1 EU/µg).
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Maus Wirt: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % SDS
Sonic Hedgehog (SHH) (Active) proteinSHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Sonic Hedgehog (SHH) proteinSHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Applikationshinweise
- Each Investigator should determine their own optimal working dilution for specific applications.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Reconstitute to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Supplied as a lyophilized powder. Sterile filtered through a 0.2 micron filter. Lyophilized from 1x PBS.
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Handhabung
- Avoid repeated freeze/thaw cycles.
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C to -80 °C for long term storage.
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- Sonic Hedgehog (SHH)
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Andere Bezeichnung
- Sonic Hedgehog
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Hintergrund
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Sonic the hedgehog homolog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog and Indian hedgehog. SHH is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain.
Alternative Names: SHH protein -
Pathways
- Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target
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