KIT Ligand Protein (KITLG) (full length)

Produktnummer ABIN935785

Recombinant KIT Ligand-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für KIT Ligand Protein (KITLG) (full length) (ABIN935785)

Target: KIT Ligand (KITLG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 98 % pure

Produktdetails

Proteineigenschaft: full length

Produktmerkmale: Purified recombinant Human SCF protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Aufreinigung: Proprietary chromatographic technique

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Kommentare:

Biological activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is

Testdurchführung:

Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water not less than 100 μg/mL.

Buffer: Lyophilized from a concentrated (1 mg/mL) solution in water containing 11 mM CH3COOH.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Antigendetails

Target: KIT Ligand (KITLG)

Andere Bezeichnung: SCF

Hintergrund: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as steel factor or c-kit ligand. SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
Alternative Names: SCF protein, MGF protein, Mast cell growth factor protein, Kitl protein, c-Kit Ligand protein, Steel Factor protein, KL-1 protein, MGF protein, C-kit ligand protein, SF protein, Mast Cell Growth Factor protein, Kit ligand Precursor protein, DKFZp686F2250, Stem Cell Factor protein

Molekulargewicht: 18.409 Da

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung