IL-18 Protein (AA 36-192)

Produktnummer ABIN8057014

Dieses Recombinant IL-18-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für IL-18 Protein (AA 36-192) (ABIN8057014)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Immunogen (Imm)

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 36-192

Verwendungszweck: Recombinant Mouse IL-18 Protein(Halo Tag)

Sequenz: Asn36-Ser192

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: IL-18

Hintergrund: Interferon-gamma inducing factor (IGIF),IL1F4,Interleukin-18(IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein(IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Molekulargewicht:

Calculated MW: 59.1 kDa

Observed MW: 58 kDa

Gen-ID: 16173

UniProt: P70380

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome