LGMN Protein

Produktnummer ABIN7964652

Dieses Recombinant LGMN-Protein wird in Leishmania tarentolae exprimiert.

Kurzübersicht für LGMN Protein (ABIN7964652)

Target: LGMN (Legumain (LGMN))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Leishmania tarentolae

Reinheit: > 90 % (SDS-PAGE)

Produktdetails

Verwendungszweck: Legumain, Asparaginyl Endopeptidase (AEP), human, recombinant, Leishmania

Produktmerkmale: Legumain is a cysteine protease with a strict specificity for cleaving after asparagine and, to a lesser extent, aspartic acid residues. Therefore, it is synonymously named the asparaginyl endopeptidase or AEP. Its very strict substrate specificity makes it an ideal enzyme for example for digestion proteomics. Human legumain is synthesized as an inactive proenzyme (56 kDa) composed of the caspase-like catalytic AEP domain (36 kDa) and a C-terminal death-domain-like prodomain. Activation to the active AEP proceeds via the pH -dependent autocatalytic release of the C-terminal prodomain at acidic pH . While prolegumain is stable at neutral pH , AEP is stable at acidic pH (≤ 6.0) but will irreversibly unfold at near neutral pH conditions. The pH -optimum of its endopeptidase activity is at pH 5.5 with a strong preference for cleaving after asparagine residues. Hydrolysis of aspartyl peptide bonds is preferentially catalyzed at more acidic pH ( pH 4.0). In addition to its well established protease activity, legumain harbors a pH -dependent ligase activity at near neutral pH .

Anwendungsinformationen

Kommentare:

Optional[Concentration Comments]: 500 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: supplied in 20 mM citric acid pH 4.0100 mM NaCl2 mM DTT

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles

Lagerung: -20 °C

Informationen zur Lagerung: store at -20 °C avoid freeze/thaw cycles

Haltbarkeit: 12 months

Antigendetails

Target: LGMN (Legumain (LGMN))

Andere Bezeichnung: Legumain

Molekulargewicht: 36 kDa

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades