LGMN Protein (His tag)

Produktnummer ABIN7320693

Dieses Recombinant LGMN-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für LGMN Protein (His tag) (ABIN7320693)

Target: LGMN (Legumain (LGMN))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses LGMN Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Legumain/LGMN Protein (His Tag)

Sequenz: Val 18-Tyr 435

Produktmerkmale: Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Not validated for activity

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 20 % Glycerol, pH 8.0.

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: LGMN (Legumain (LGMN))

Andere Bezeichnung: Legumain/LGMN

Hintergrund: AEP,Asparaginyl endopeptidase,Legumain,Lgmn,Protease cysteine 1,Prsc1,Mouse Legumain,also known as LGMN, is a cysteine protease belonging to peptidase family C13 and is expressed in kidney and placenta abundantly. LGMN has a strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. The mammalian legumain is involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. It plays a role in the regulation of cell proliferation via its role in EGFR degradation. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Mammalian legumain is inhibited by iodoacetamide and maleimides. Legumain activation appears to be autocatalytic and can be triggered by acidic pH .

Molekulargewicht:

Calculated MW: 48.7 kDa

Observed MW: 60 kDa

NCBI Accession: NP_035305

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades