IGF1R Protein (AA 763-931)

Produktnummer ABIN7939184

Recombinant IGF1R-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für IGF1R Protein (AA 763-931) (ABIN7939184)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Screening Assay (ScA), Functional Studies (Func), Activity Assay (AcA)

Produktdetails

Proteineigenschaft: AA 763-931

Verwendungszweck: Recombinant IGF1R (763-931) protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant IGF1R (763-931) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: IGF1R

Hintergrund:

Protein Background: IGF1R, a receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1) and binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2). Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 leads to recruitment of Ras and activation of the ras-MAPK pathway.

Short Description: Recombinant IGF1R (763-931) protein that includes amino acids 763-931 of human IGF1R protein (accession number NP_000866.1) was expressed in a baculovirus expression system, and contains an N-terminal FLAG tag. The molecular weight of the protein is 20.4 kDa

Aliases: Insulin Like Growth Factor 1 Receptor

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie