HMGB1 Protein (BoxAB, C-Tailless)

Produktnummer ABIN7862612

Recombinant HMGB1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für HMGB1 Protein (BoxAB, C-Tailless) (ABIN7862612)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activation (Act), Cellular Assay (CA)

Reinheit: > 95 % It contains no nucleic acids.

Produktdetails

Proteineigenschaft: BoxAB, C-Tailless

Verwendungszweck: BoxAB (Tailless-HMGB1) is a recombinant fragment of HMGB1 including both DNA-binding domains, BoxA and BoxB, lacking the acidic C-terminal tail.

Sequenz: BoxAB from HMGB1 consists of 176 amino acids: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVV

Produktmerkmale: BoxAB from HMGB1 includes both BoxA and BoxB domains connected as in the natural sequence but lacks the negatively charged C-terminal tail. This tailless form retains the structural integrity and receptor-binding capacity of full-length HMGB1, while showing altered functional behavior in redox-dependent signaling and immune activation.
BoxAB is ideal for studying the mechanisms of HMGB1-mediated inflammation, receptor binding (RAGE, TLR2, TLR4), and DNA interaction without interference from the acidic tail. The protein is fully compatible with in vitro biochemical assays, cell culture studies, and receptor-ligand binding analyses.
The product is produced in E.coli from an expression plasmid coding for the human HMGB1 sequence (amino acids 1–176) without any tags or modifications, ensuring natural folding and charge distribution.

Aufreinigung: Purified. Oxidation of cysteine 106 makes the protein inactive. To avoid cysteine oxidation DTT 0.5 mM is added during protein purification.

Endotoxin-Niveau: LPS-free: Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: The lyophilized protein should be reconstituted in distilled water, resulting in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.

Buffer: The protein is lyophilized from 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
The product contains contains < 0.006% v/v of Triton X-114 due to LPS removal. Remaining traces can be removed upon request.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: 2-8°C when lyophilized. The protein once reconstituted with water can be stored frozen (-20°C).

Antigendetails

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-mobility group protein B1 (HMGB1)

Hintergrund: HMGB1 is a nuclear protein that is released passively by necrotic cells, retained by apoptotic cells, and secreted actively by inflammatory cells.

Molekulargewicht: 20.0 kDa

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome