Cathepsin L Protein (AA 18-333) (His tag)

Produktnummer ABIN7597376

Dieses Recombinant Cathepsin L-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für Cathepsin L Protein (AA 18-333) (His tag) (ABIN7597376)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Produktdetails

Proteineigenschaft: AA 18-333

Aufreinigungstag / Konjugat: Dieses Cathepsin L Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant human CTSL Protein with C-terminal 10xHis tag

Sequenz: CTSL(Thr18-Val333) 10xHis tag

Anwendungsinformationen

Applikationshinweise: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Kommentare:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Andere Bezeichnung: CTSL

Hintergrund: MEP, CATL, CTSL1
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Additionally, this protein cleaves the S1 subunit of the SARS-CoV-2 spike protein, which is necessary for entry of the virus into the cell. [provided by RefSeq, Aug 2020]

Molekulargewicht: predicted molecular mass of 37.2 kDa after removal of the signal peptide. The apparent molecular mass of CTSL-His is 35-55 kDa due to glycosylation.

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades