Flagellin Protein (FliC) (AA 1-495, active-Mutant)

Produktnummer ABIN7566155

Dieses Recombinant Flagellin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Flagellin Protein (FliC) (AA 1-495, active-Mutant) (ABIN7566155)

Target: Flagellin (FliC)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Salmonella typhimurium

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % (SDS-PAGE)

Produktdetails

Proteineigenschaft: AA 1-495, active-Mutant

Verwendungszweck: Flagellin (rec.) (untagged) (highly active)

Kreuzreaktivität: Human, Maus

Produktmerkmale: Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).

Endotoxin-Niveau: <0.001EU/μg purified protein (LAL test, Lonza).

Biological Activity Comment: Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 1 mg/mL after reconstitution.

Konzentration: 1 mg/mL

Buffer: Contains PBS.

Handhabung: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

Antigendetails

Target: Flagellin (FliC)

Andere Bezeichnung: Flagellin

Hintergrund:

FliC

Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

Molekulargewicht: ~54kDa (SDS-PAGE)

UniProt: P06179

Pathways: Inflammasome