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Flagellin Protein (FliC) (AA 1-495, active-Mutant)

Dieses Recombinant Flagellin-Protein wird in Escherichia coli (E. coli) produziert.
Produktnummer ABIN7566155

Kurzübersicht für Flagellin Protein (FliC) (AA 1-495, active-Mutant) (ABIN7566155)

Target

Alle Flagellin (FliC) Proteine anzeigen
Flagellin (FliC)

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Salmonella typhimurium

Quelle

  • 7
  • 5
  • 2
Escherichia coli (E. coli)

Reinheit

>95 % (SDS-PAGE)
  • Proteineigenschaft

    AA 1-495, active-Mutant

    Verwendungszweck

    Flagellin (rec.) (untagged) (highly active)

    Kreuzreaktivität

    Human, Maus

    Produktmerkmale

    Native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495).

    Endotoxin-Niveau

    <0.001EU/μg purified protein (LAL test, Lonza).

    Biological Activity Comment

    Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (rec.) (untagged) (highly active) at a concentration of 5-50ng/ml and in vivo at a starting concentration of 0.1-1μg/mouse.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    1 mg/mL after reconstitution.

    Konzentration

    1 mg/mL

    Buffer

    Contains PBS.

    Handhabung

    After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Short Term Storage: +4°C

    Long Term Storage: -20°C

    Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.

  • Target

    Flagellin (FliC)

    Andere Bezeichnung

    Flagellin

    Hintergrund

    FliC

    Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).

    Molekulargewicht

    ~54kDa (SDS-PAGE)

    UniProt

    P06179

    Pathways

    Inflammasome
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