SLC16A1 Protein (AA 1-493) (His tag)

Produktnummer ABIN7563442

Dieses Recombinant SLC16A1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für SLC16A1 Protein (AA 1-493) (His tag) (ABIN7563442)

Target: SLC16A1 (Solute Carrier Family 16, Member 1 (Monocarboxylic Acid Transporter 1) (SLC16A1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Produktdetails

Proteineigenschaft: AA 1-493

Aufreinigungstag / Konjugat: Dieses SLC16A1 Protein ist gelabelt mit His tag.

Verwendungszweck: Predefined custom protein recombinant Slc16a1 Protein expressed in mammalian cells.

Sequenz: MPPAIGGPVG YTPPDGGWGW AVLVGAFISI GFSYAFPKSI TVFFKEIEVI FSATTSEVSW ISSIMLAVMY AGGPISSILV NKYGSRPVMI AGGCLSGCGL IAASFCNTVQ ELYLCIGVIG GLGLAFNLNP ALTMIGKYFY KKRPLANGLA MAGSPVFLST LAPLNQAFFD IFDWRGSFLI LGGLLLNCCV AGSLMRPIGP EQVKLEKLKS KESLQEAGKS DANTDLIGGS PKGEKLSVFQ TINKFLDLSL FTHRGFLLYL SGNVVMFFGL FTPLVFLSSY GKSKDFSSEK SAFLLSILAF VDMVARPSMG LAANTKWIRP RIQYFFAASV VANGVCHLLA PLSTTYVGFC VYAGVFGFAF GWLSSVLFET LMDLIGPQRF SSAVGLVTIV ECCPVLLGPP LLGRLNDMYG DYKYTYWACG VILIIAGIYL FIGMGINYRL LAKEQKAEEK QKREGKEDEA STDVDEKPKE TMKAAQSPQQ HSSGDPTEEE SPV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: SLC16A1 (Solute Carrier Family 16, Member 1 (Monocarboxylic Acid Transporter 1) (SLC16A1))

Andere Bezeichnung: Slc16a1

Hintergrund: Monocarboxylate transporter 1 (MCT 1) (Solute carrier family 16 member 1),FUNCTION: Bidirectional proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular  pH (By similarity). The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity). Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (PubMed:22522610, PubMed:24367518). Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart. Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 302-Asp is essential for the conformational transition (PubMed:32946811). {ECO:0000250|UniProtKB:P53985, ECO:0000250|UniProtKB:P53987, ECO:0000269|PubMed:22522610, ECO:0000269|PubMed:24367518, ECO:0000269|PubMed:32946811}.

Molekulargewicht: 53.3 kDa

UniProt: P53986

AlphaFold: P53986

Pathways: Warburg Effekt