PD-L1 Protein (His tag)

Produktnummer ABIN7535911

Recombinant PD-L1-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für PD-L1 Protein (His tag) (ABIN7535911)

Target: PD-L1 (CD274 (PD-L1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: HEK-293 Cells

Reinheit: > 95 % by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PD-L1 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Rat B7-H1/PD-L1/CD274 Protein

Sequenz: AFTITAPKDL YVVEYGSNVT MECRFPVEQK LDLLALVVYW EKEDKEVIQF VEGEEDLKPQ HSSFRGRAFL PKDQLLKGNA VLQITDVKLQ DAGVYCCMIS YGGADYKRIT LKVNAPYRKI NQRISMDPAT SEHELMCQAE GYPEAEVIWT NSDHQSLSGE TTVTTSQTEE KLLNVTSVLR VNATANDVFH CTFWRVHSGE NHTAELIIPE LPVPRLPHNR T

Spezifität: Ala18-Thr238

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: <0.1EU/μg

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 at 10 μg/mL (100 μL/well) can bind Biotinylated Rat PD-1 with a linear range of 0.0195-2.16 μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Antigendetails

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: B7-H1/PD-L1/CD274

Hintergrund:

Description: This protein is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation.

Name: Pdcd1lg1, RGD1566211,CD274

Gen-ID: 499342

UniProt: D4AE25

Pathways: Cancer Immune Checkpoints