Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

PD-L1 Protein (AA 19-238) (His tag,AVI tag,Biotin)

PD-L1 Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Produktnummer ABIN2870558
  • Target Alle PD-L1 Proteine anzeigen
    PD-L1 (CD274 (PD-L1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 19-238
    Spezies
    • 41
    • 18
    • 6
    • 5
    • 4
    • 3
    • 1
    Human
    Quelle
    • 46
    • 9
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses PD-L1 Protein ist gelabelt mit His tag,AVI tag,Biotin.
    Marke
    MABSol®,PrecisionAvi
    Sequenz
    AA 19-238
    Spezifität
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
    Produktmerkmale
    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 27.8 kDa. The protein migrates as 38-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Reinheit
    >95 % as determined by SDS-PAGE.
    Endotoxin-Niveau
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product PD-L1 Protein
  • Kommentare

    Ready-to-use AvitagTM biotinylated protein:
    The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

    This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4
    Handhabung
    Please avoid repeated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Son, Park, Seo, Lee, Heo, Kim: "A small-sized protein binder specific for human PD-1 effectively suppresses the tumour growth in tumour mouse model." in: Journal of drug targeting, pp. 1-9, (2019) (PubMed).

    Ganesan, Ahmed, Okoye, Arutyunova, Babu, Turnbull, Kundu, Shields, Agopsowicz, Xu, Tabana, Srivastava, Zhang, Moon, Belovodskiy, Hena, Kandadai, Hosseini, Hitt, Walker, Smylie, West, Siraki, Lemieux et al.: "Comprehensive in vitro characterization of PD-L1 small molecule inhibitors. ..." in: Scientific reports, Vol. 9, Issue 1, pp. 12392, (2019) (PubMed).

    Arlauckas, Garris, Kohler, Kitaoka, Cuccarese, Yang, Miller, Carlson, Freeman, Anthony, Weissleder, Pittet: "In vivo imaging reveals a tumor-associated macrophage-mediated resistance pathway in anti-PD-1 therapy." in: Science translational medicine, Vol. 9, Issue 389, (2018) (PubMed).

  • Target
    PD-L1 (CD274 (PD-L1))
    Andere Bezeichnung
    PD-L1 (PD-L1 Produkte)
    Synonyme
    B7-H Protein, B7H1 Protein, PD-L1 Protein, PDCD1L1 Protein, PDCD1LG1 Protein, PDL1 Protein, A530045L16Rik Protein, B7h1 Protein, Pdcd1l1 Protein, Pdcd1lg1 Protein, Pdl1 Protein, RGD1566211 Protein, CD274 molecule Protein, CD274 antigen Protein, programmed cell death 1 ligand 2 Protein, CD274 Protein, Cd274 Protein, PDCD1LG2 Protein
    Hintergrund
    Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
    Molekulargewicht
    27.8 kDa
    NCBI Accession
    NP_054862
    Pathways
    Cancer Immune Checkpoints
Sie sind hier:
Kundenservice