PD-L1 Protein (His tag)

Produktnummer ABIN7519763

Dieses Recombinant PD-L1-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für PD-L1 Protein (His tag) (ABIN7519763)

Target: PD-L1 (CD274 (PD-L1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % by SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses PD-L1 Protein ist gelabelt mit His tag.

Verwendungszweck: Active Recombinant Mouse B7-H1/PD-L1/CD274 Protein

Sequenz: MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRT

Spezifität: Met1-Thr238

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-L1 at 10 μg/mL (100 μL/well) can bind Recombinant Mouse PD-1 with a linear range of 1.5-5 μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: B7-H1/PD-L1/CD274

Hintergrund:

Description: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1). PD-L1/B7-H1 is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma.

Name: PD-, B7h1, Pdl1, Pdcd1l, Pdcd1l1, Pdcd1lg1, A530045L16Rik,CD274

Gen-ID: 60533

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints