CD14 Protein (CD14) (His tag)
Kurzübersicht für CD14 Protein (CD14) (His tag) (ABIN7534605)
Target
Alle CD14 Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Reinheit
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Aufreinigungstag / Konjugat
- Dieses CD14 Protein ist gelabelt mit His tag.
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Verwendungszweck
- Active Recombinant Human CD14 Protein
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Sequenz
- TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV PAC
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Spezifität
- Thr20-Cys352
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Sterilität
- 0.22 μm filtered
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Endotoxin-Niveau
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED50 for this effect is 5.7-23 ng/mL.
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CD14 (CD14) (AA 16-345) protein (His tag)
CD14 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 20-344) protein (His tag)CD14 Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD14 (CD14) (AA 1-375) protein (GST tag)CD14 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
CD14 (CD14) (AA 20-352) protein (His tag)CD14 Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
CD14 (CD14) (AA 20-349) protein (His tag)CD14 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
CD14 (CD14) protein (His tag,Fc Tag)CD14 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
CD14 (CD14) protein (His tag)CD14 Spezies: Ratte Wirt: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
CD14 (CD14) protein (Fc Tag)CD14 Spezies: Ratte Wirt: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- CD14
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Andere Bezeichnung
- CD14
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Hintergrund
- CD14
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Gen-ID
- 929
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UniProt
- P08571
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Pathways
- TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Target
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