Pro BDNF Protein (V66M)

Produktnummer ABIN7482108

Dieses Recombinant Pro BDNF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Pro BDNF Protein (V66M) (ABIN7482108)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Cell Culture (CC)

Reinheit: ≥98 % (HPLC)

Produktdetails

Proteineigenschaft: V66M

Verwendungszweck: Recombinant human Val66Met proBDNF (cleavage resistant) protein

Sequenz: MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHMI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVA GHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR (the prodomain is shown in bold, underlined are the double point mutations preventing cleavage (AG). The Val66Met mutation is underlined in the sequence).

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors

Endotoxin-Niveau: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor that bind p75NTR as well as TrkB receptors1,2 and supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75NTR and sortilin, and to be involved in long-term potentiation (LTP) stage of the memory-related modifications in synaptic transmission10.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 0.1 - 10 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Andere Bezeichnung: proBDNF

Hintergrund: A cleavage-resistant human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling

Molekulargewicht: 51.45 kDa (dimer)