Pro BDNF Protein (Biotin)

Produktnummer ABIN7491800

Dieses Recombinant Pro BDNF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Pro BDNF Protein (Biotin) (ABIN7491800)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Cell Culture (CC), Live Cell Imaging (LCI)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Pro BDNF Protein ist gelabelt mit Biotin.

Verwendungszweck: human proBDNF-Biotin

Sequenz: MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHVI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVAGHSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR (the prodomain is shown in bold, underlined are the double point mutations preventing cleavage)

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors

Produktmerkmale: LC-Biotin. The extent of labeling is 1-3 Molecules of biotin per one molecule of human proBDNF, linked via primary amines.

Biological Activity Comment: proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons expressing both p75 and sortilin.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 0.1 - 10 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Andere Bezeichnung: proBDNF

Hintergrund: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)

Molekulargewicht: ~52 kDa (dimer)