Pro BDNF Protein

Produktnummer ABIN7491767

Dieses Recombinant Pro BDNF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Pro BDNF Protein (ABIN7491767)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), In vivo Studies (in vivo), In vitro Assay (in vitro)

Reinheit: ≥98 % (HPLC)

Produktdetails

Verwendungszweck: Recombinant mouse proBDNF protein

Sequenz: MAPMKEVNVH GQGNLAYPGV RTHGTLESVN GPRAGSRGLT TTSLADTFEH VIEELLDEDQ KVRPNEENHK DADLYTSRVM LSSQVPLEPP LLFLLEEYKN YLDAANMSMR VRRHSDPARR GELSVCDSIS EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG CRGIDKRHWN SQCRTTQSYV RALTMDSKKR IGWRFIRIDT SCVCTLTIKR GR (the prodomain is shown in bold)

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors

Endotoxin-Niveau: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 0.2 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Andere Bezeichnung: proBDNF

Hintergrund: A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex

Molekulargewicht: 52.4 kDa (dimer)