SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (Pro13Leu-Mutant) (His tag)

Produktnummer ABIN7490687

Produktdetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Protein-Typ: Recombinant

Proteineigenschaft: Pro13Leu-Mutant

Spezies: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Nucleocapsid Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant SARS-CoV-2 Nucleocapsid (P13L, E31del, R32del, S33del, R203K, G204R) protein with C-terminal 6xHis tag

Spezifität: Nucleocapsid (P13L, E31del, R32del, S33del, R203K, G204R) (Met1-Ala419) 6xHis tag

Produktmerkmale: Extracellular Domain Protein

Aufreinigung: Purified from cell culture supernatant by affinity chromatography

Reinheit: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Haltbarkeit: 12 months

Antigendetails

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Andere Bezeichnung: SARS-CoV-2 N (SARS-CoV-2 N Produkte)

Substanzklasse: Viral Protein

Hintergrund: Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.

Molekulargewicht: predicted molecular mass of 46.3 kDa after removal of the signal peptide. The apparent molecular mass of Nucleocapsid (D63G, R203M, D377Y)-His is 55-70 kDa due to glycosylation.

UniProt: P0DTC9