SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag)
Kurzübersicht für SARS-CoV-2 Spike S1 Protein (Fc-Avi Tag) (ABIN7275552)
Target
Alle SARS-CoV-2 Spike S1 Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Aufreinigungstag / Konjugat
- Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit Fc-Avi Tag.
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Verwendungszweck
- SARS Spike S1 Protein
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Sequenz
- Ser14-Arg667
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Produktmerkmale
- Recombinant SARS spike S1 protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser14-Arg667.
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Sterilität
- 0.22 μm filtered
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Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human ACE2 at 2μg/ml (100μl/Well).Dose response curve for SARS Spike S1, hFc Tag with the EC50 of 0.39μg/ml determined by ELISA. See testing image for detail.
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SARS-CoV-2 Spike S1 protein (His tag)
Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func
SARS-CoV-2 Spike S1 (B.1.1.529 - Omicron) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
SARS-CoV-2 Spike S1 (N501Y) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (E484K), (K417N), (N501Y) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.4 - Omicron), (BA.5 - Omicron) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.2.12.1 - Omicron) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (B.1.617.2 - delta) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (D614G) protein (His-Avi Tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (P.1 - gamma) protein (His tag)Spezies: SARS Coronavirus-2 (SARS-CoV-2) Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- SARS-CoV-2 Spike S1
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Andere Bezeichnung
- SARS-CoV Spike S1
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Hintergrund
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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Molekulargewicht
- 100.6 kDa. Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result.
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UniProt
- P59594
Target
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