PRNP Protein (AA 23-230) (Fc Tag)
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- Target Alle PRNP Proteine anzeigen
- PRNP (Prion Protein (PRNP))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 23-230
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses PRNP Protein ist gelabelt mit Fc Tag.
- Verwendungszweck
- Human PRNP Protein
- Sequenz
- Lys23-Ser230
- Produktmerkmale
- Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys23-Ser230.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human PRNP, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
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Prion Protein (PRNP) (AA 23-230) protein (Fc Tag)
PRNP Spezies: Maus Wirt: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Prion Protein (PRNP) (AA 1-253) protein (GST tag)PRNP Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Prion Protein (PRNP) (AA 90-230) protein (Fc Tag)PRNP Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Prion Protein (PRNP) (AA 23-229) protein (Fc Tag)PRNP Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Prion Protein (PRNP) (AA 29-231) protein (His tag)PRNP Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
Prion Protein (PRNP) proteinPRNP Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % ELISA, SDS, WB, IP
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- PRNP (Prion Protein (PRNP))
- Andere Bezeichnung
- PRNP (PRNP Produkte)
- Hintergrund
- Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression.
- Molekulargewicht
- 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Tris-Bis PAGE result.
- UniProt
- P04156
- Pathways
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
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