TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
Protein-Typ
Recombinant
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses TNFRSF4 Protein ist gelabelt mit His-Avi Tag.
Sequenz
Val20-Pro211
Reinheit
> 95% as determined by Tris-Bis PAGE,> 92% as determined by HPLC
Sterilität
0.22 μm filtered
Endotoxin-Niveau
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse OX-40 Ligand, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Mouse OX-40, His Tag with the EC50 3.59μg/ml determined by ELISA. See testing image for detail.
TNFRSF4
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
>90 % as determined by SDS-PAGE.
Active
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Buffer
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Haltbarkeit
12 months
Target
TNFRSF4
(Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))
CD134, OX40, OX40L receptor, TNFRSF4, ACT-135, Ly-70, OX40 homologue, TXGP1L, IMD16,Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Molekulargewicht
24.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.