IL-15 Protein (AA 49-162)

Produktnummer ABIN7274953

Dieses Recombinant IL-15-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-15 Protein (AA 49-162) (ABIN7274953)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC

Produktdetails

Proteineigenschaft: AA 49-162

Verwendungszweck: Human IL-15 Protein

Sequenz: Asn49-Ser162

Produktmerkmale: Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using CTLL­2 cells. The ED50 for this effect is 0.1 ­ 0.3 ng/mL. The specific activity of recombinant human IL­15 is > 1 x 10^7 units/mg, which is calibrated against the human IL­15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Andere Bezeichnung: IL-15

Hintergrund: Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

Molekulargewicht: 12.8 kDa same as Tris-Bis PAGE result.

UniProt: P40933

Pathways: JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process