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IL-15 Protein (AA 49-162)

Dieses Recombinant IL-15-Protein wird in Escherichia coli (E. coli) exprimiert.
Produktnummer ABIN7274953

Kurzübersicht für IL-15 Protein (AA 49-162) (ABIN7274953)

Target

Alle IL-15 (IL15) Proteine anzeigen
IL-15 (IL15) (Interleukin 15 (IL15))

Protein-Typ

Recombinant

Spezies

  • 27
  • 9
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Quelle

  • 39
  • 10
  • 9
  • 4
  • 1
  • 1
Escherichia coli (E. coli)

Reinheit

>95 % as determined by Tris-Bis PAGE, > 95 % as determined by SEC-HPLC
  • Proteineigenschaft

    AA 49-162

    Verwendungszweck

    Human IL-15 Protein

    Sequenz

    Asn49-Ser162

    Produktmerkmale

    Recombinant Human IL-15 Protein is expressed from E.coli without tag., It contains Asn49-Ser162.

    Sterilität

    0.22 μm filtered

    Endotoxin-Niveau

    Less than 0.05 EU per μg by the LAL method.

    Biological Activity Comment

    Measured in a cell proliferation assay using CTLL­2 cells. The ED50 for this effect is 0.1 ­ 0.3 ng/mL. The specific activity of recombinant human IL­15 is > 1 x 10^7 units/mg, which is calibrated against the human IL­15 reference standard (NIBSC code: 95/554). Test result was comparable to standard batch.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4) / 20 mM Tris, 150 mM NACI ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    IL-15 (IL15) (Interleukin 15 (IL15))

    Andere Bezeichnung

    IL-15

    Hintergrund

    Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases. IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.

    Molekulargewicht

    12.8 kDa same as Tris-Bis PAGE result.

    UniProt

    P40933

    Pathways

    JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process
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