IL-4 Protein (AA 25-153) (His-Avi Tag,Biotin)

Produktnummer ABIN7274951

Produktdetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-153

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL-4 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human IL-4 Protein

Sequenz: His25-Ser153

Produktmerkmale: Recombinant Biotinylated Human IL-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His25-Ser153.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-4 R alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human IL-4, His Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4 (IL4 Produkte)

Hintergrund: Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation.In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor.

Molekulargewicht: 17.8 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation