IL3RA Protein (AA 19-305) (His tag)

Produktnummer ABIN7274943

Produktdetails

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-305

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL3RA Protein ist gelabelt mit His tag.

Verwendungszweck: Human IL-3 R alpha/CD123 Protein

Sequenz: Thr19-Arg305

Produktmerkmale: Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr19-Arg305.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. The affinity constant of 0.276 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Andere Bezeichnung: IL-3 R alpha (IL3RA Produkte)

Hintergrund: Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.

Molekulargewicht: 33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signalweg