IL-18 Protein (AA 37-193)
Kurzübersicht für IL-18 Protein (AA 37-193) (ABIN7274880)
Target
Alle IL-18 (IL18) Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Proteineigenschaft
- AA 37-193
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Verwendungszweck
- Human IL-18 Protein
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Sequenz
- Tyr37-Asp193
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Produktmerkmale
- Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.
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Sterilität
- 0.22 μm filtered
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Endotoxin-Niveau
- Less than 0.02 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 1.21nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- IL-18 (IL18) (Interleukin 18 (IL18))
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Andere Bezeichnung
- IL-18
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Hintergrund
- Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
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Molekulargewicht
- 18.2 kDa same as Tris-Bis PAGE result.
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UniProt
- Q14116
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target
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