IL-18 Protein (AA 37-193)
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- Target Alle IL-18 (IL18) Proteine anzeigen
- IL-18 (IL18) (Interleukin 18 (IL18))
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 37-193
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Verwendungszweck
- Human IL-18 Protein
- Sequenz
- Tyr37-Asp193
- Produktmerkmale
- Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193.
- Reinheit
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 0.02 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-18 at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. The affinity constant of 1.21nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 18 (IL18) (AA 37-193) protein (Biotin)
IL18 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Interleukin 18 (IL18) (AA 37-193) protein (His-IF2DI Tag)IL18 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 18 (IL18) (AA 36-192) protein (His tag)IL18 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Interleukin 18 (IL18) (AA 1-193) protein (GST tag)IL18 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 18 (IL18) protein (GST tag)IL18 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 85 % as determined by reducing SDS-PAGE.
Interleukin 18 (IL18) protein (His tag)IL18 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE
Interleukin 18 (IL18) (AA 37-197) protein (Fc Tag)IL18 Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 18 (IL18) (AA 37-197) protein (mFc Tag)IL18 Spezies: Human Wirt: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in 20 mM PB, 250 mM NaCl, 50 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- IL-18 (IL18) (Interleukin 18 (IL18))
- Andere Bezeichnung
- IL-18 (IL18 Produkte)
- Hintergrund
- Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
- Molekulargewicht
- 18.2 kDa same as Tris-Bis PAGE result.
- UniProt
- Q14116
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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