GP6 Protein (AA 21-267) (His tag)

Produktnummer ABIN7274760

Dieses Recombinant GP6-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für GP6 Protein (AA 21-267) (His tag) (ABIN7274760)

Target: GP6 (Glycoprotein VI (Platelet) (GP6))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 21-267

Aufreinigungstag / Konjugat: Dieses GP6 Protein ist gelabelt mit His tag.

Verwendungszweck: Human GPVI Protein

Sequenz: Gln21-Lys267

Produktmerkmale: Recombinant Human GPVI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Lys267.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GPVI, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 14.8ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GP6 (Glycoprotein VI (Platelet) (GP6))

Andere Bezeichnung: GPVI

Hintergrund: Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair. Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). GPVI is activated by collagen in the subendothelial matrix, by fibrin and fibrinogen in the thrombus, and by a remarkable number of other ligands.

Molekulargewicht: 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

UniProt: Q9HCN6-1