GDNF Protein (AA 78-211)

Produktnummer ABIN7274722

Dieses Recombinant GDNF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für GDNF Protein (AA 78-211) (ABIN7274722)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 78-211

Verwendungszweck: Human GDNF Protein

Sequenz: Ser78-Ile211

Produktmerkmale: Recombinant Human GDNF Protein is expressed from E.coli without tagIt contains Ser78-Ile211.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GDNF, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GFR alpha 1, His Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Andere Bezeichnung: GDNF

Hintergrund: Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons.

Molekulargewicht: 15.06 kDa same as Tris-Bis PAGE result.

UniProt: P39905-1

Pathways: RTK Signalweg, Synaptic Membrane, Tube Formation, Autophagie, Smooth Muscle Cell Migration