CD3E & CD3G (AA 23-120) protein (His tag)

Produktnummer ABIN7274144

Recombinant -Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CD3E & CD3G (AA 23-120) protein (His tag) (ABIN7274144)

Target: CD3E & CD3G

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 23-120

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

Sequenz: Asp23-Glu120(CD3E) & Gln23-Asn106(CD3G)

Produktmerkmale: Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp23-Glu120(CD3E)&Gln23-Asn106(CD3G).

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA. The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD3E & CD3G

Andere Bezeichnung: CD3E&CD3G

Hintergrund: T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.

Molekulargewicht: 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.