BAFF Protein (AA 134-285) (Fc Tag)

Produktnummer ABIN7273914

Produktdetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 134-285

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses BAFF Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human BAFF/TNFSF13B/CD257 Protein

Sequenz: Ala134-Leu285

Produktmerkmale: Recombinant Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala134-Leu285.

Reinheit: > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human BCMA Trimer, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 17ng/ml determined by ELISA. The affinity constant of 5.83 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Andere Bezeichnung: BAFF (TNFSF13B Produkte)

Hintergrund: B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

Molekulargewicht: 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.

UniProt: Q9Y275-1

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response