TGFB1 Protein

Produktnummer ABIN7321247

Dieses Recombinant TGFB1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für TGFB1 Protein (ABIN7321247)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: HEK-293 Cells

Reinheit: > 90 % as determined by SDS-PAGE

Produktdetails

Verwendungszweck: Recombinant Rat TGFB1/TGF-beta 1 Protein (Active)

Sequenz: Ala279-Ser390

Produktmerkmale: A DNA sequence encoding the rat TGFB1 (P17246)(Ala279-Ser390) was expressed. Rat and Mouse mature TGFB1 sequences are identical.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to inhibit cell proliferation of Mv-1-lu mink lung epithelial cells. The ED50 for this effect is 0.2-0.8 ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGFB1/TGF-beta 1

Hintergrund:

Background: TGF-beta 1 is a member of the transforming growth factor beta (TGF-beta) family. The transforming growth factor-beta family of polypeptides are involved in the regulation of cellular processes, including cell division, differentiation, motility, adhesion and death. TGF-beta 1 positively and negatively regulates many other growth factors. It inhibits the secretion and activity of many other cytokines including interferon-γ, tumor necrosis factor-alpha and various interleukins. It can also decrease the expression levels of cytokine receptors. Meanwhile, TGF-beta 1 also increases the expression of certain cytokines in T cells and promotes their proliferation, particularly if the cells are immature. TGF-beta 1 also inhibits proliferation and stimulates apoptosis of B cells, and plays a role in controlling the expression of antibody, transferrin and MHC class II proteins on immature and mature B cells. As for myeloid cells, TGF-beta 1can inhibit their proliferation and prevent their production of reactive oxygen and nitrogen intermediates. However, as with other cell types, TGF-beta 1 also has the opposite effect on cells of myeloid origin. TGF-beta 1 is a multifunctional protein that controls proliferation, differentiation and other functions in many cell types. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Once cells lose their sensitivity to TGF-beta1-mediated growth inhibition, autocrine TGF-beta signaling can promote tumorigenesis. Elevated levels of TGF-beta1 are often observed in advanced carcinomas, and have been correlated with increased tumor invasiveness and disease progression.

Synonym: TGF-beta 1,TGFB,TGFbeta

Molekulargewicht: 12.8 kDa

UniProt: P17246

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints