Thrombospondin 1 Protein (THBS1) (His tag)

Produktnummer ABIN7319437

Dieses Recombinant Thrombospondin 1-Protein wird in Human Cells exprimiert.

Kurzübersicht für Thrombospondin 1 Protein (THBS1) (His tag) (ABIN7319437)

Target: Thrombospondin 1 (THBS1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human Cells

Reinheit: > 70 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses Thrombospondin 1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Thrombospondin-1/THBS1 Protein (His Tag)(Active)

Sequenz: Asn19-Pro1170

Produktmerkmale: Recombinant Human Thrombospondin-1 is produced by our Mammalian expression system and the target gene encoding Asn19-Pro1170 is expressed with a 10His tag at the C-terminus.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Rhesus macaque CD47-Fc(Cat: PKSQ050076) at 10μg/ml(100 μl/well) can bind Human THBS1-His.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: Thrombospondin-1/THBS1

Hintergrund:

Background: Thrombospondin-1 (TSP-1) is a 150-180 kDa calcium-sensitive protein that is secreted as a disulfide-linked homotrimer. TSP-1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N-terminal Laminin G-like globular domain, an extended central region with one vWFC domain, 3 TSP type 1domains, 2 EGF-like domains, and 8 TSP type3 domains, and a globular TSP C-terminal domain. Distinct regions of TSP-1 have been associated with binding to particular ECM or cellular molecules. TSP-1 counteracts the angiogenic, hypotensive, and antithrombotic effects of nitric oxide (NO). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells(EC), and destabilizes adhesive contacts between EC. TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF-beta and inducing release of the active cytokine from the latency associated peptide (LAP).

Synonym: Thrombospondin-1, THBS1, TSP, TSP1

Molekulargewicht: 129.2 kDa

UniProt: P07996

Pathways: Autophagie