Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST)

Produktnummer ABIN7317056

Dieses Recombinant Ret Proto-Oncogene-Protein wird in Baculovirus infected Insect Cells produziert.

Kurzübersicht für Ret Proto-Oncogene Protein (RET) (AA 658-1114) (His-GST) (ABIN7317056)

Target: Ret Proto-Oncogene (RET)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 658-1114

Aufreinigungstag / Konjugat: Dieses Ret Proto-Oncogene Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human RET Kinase Protein (aa 658-1114, His & GST Tag)

Sequenz: His 658-Ser 1114

Produktmerkmale: A DNA sequence encoding the cytoplasmic domain of human RET (P07949-1) (His 658-Ser 1114) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 17 nmol/min/mg using synthetic TRK-C-derived Peptide (R11-VYSTDYYRLFNPS) as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: Ret Proto-Oncogene (RET)

Andere Bezeichnung: RET Kinase

Hintergrund: CDHF12,CDHR16,HSCR1,MEN2A,MEN2B,MTC1,PTC,RET-ELE1,RET51,RET proto-oncogene, also known as RET, is a cell-surface molecule that transduce signals for cell growth and differentiation. It contains 1 cadherin domain and 1 protein kinase domain. RET proto-oncogene belongs to the protein kinase superfamily, tyr protein kinase family. RET proto-oncogene is involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates both cell death/survival balance and positional information. RET is required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, promotes the formation of Peyer's patch-like structures, modulates cell adhesion via its cleavage, involved in the development of the neural crest. RET proto-oncogene is active in the absence of ligand, triggering apoptosis. RET acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. It also regulates nociceptor survival and size, triggers the differentiation of rapidly adapting (RA) mechanoreceptors, mediated several diseases such as neuroendocrine cancers.

Molekulargewicht:

Calculated MW: 76.7 kDa

Observed MW: 70 kDa

Gen-ID: 5979

UniProt: P07949

Pathways: RTK Signalweg, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation