IL-10 Protein (AA 19-178) (His tag)

Produktnummer ABIN7320571

Dieses Recombinant IL-10-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL-10 Protein (AA 19-178) (His tag) (ABIN7320571)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 19-178

Aufreinigungstag / Konjugat: Dieses IL-10 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse Interleukin-10/IL-10 Protein

Sequenz: Ser19-Ser178

Produktmerkmale: Recombinant Mouse Interleukin-10 is produced by our E.coli expression system and the target gene encoding Ser19-Ser178 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce MC/9-2 cells proliferation.The ED₅₀ for this effect is <1 ng/mL.The specific activity of recombinant mouse IL-10 is > 1 x 10⁶ IU/mg.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: Interleukin-10/IL-10

Hintergrund: Interleukin,IL-,CSIF,If2a,Il-10,IL10A,TGIF,Interleukin-10,IL10,Cytokine synthesis inhibitory factor,N/A,CSIF,If2a,Il-10,Mouse Il10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. Many viruses encode viral members of the IL-10 family, such as Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV).Its main function is inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Although human and mouse IL-10 are 81 % identical at the nucleotide and amino acid level, mouse IL-10 is species-specific and does not act on human cells. Interestingly, Human IL-10 is active on mouse cells.

Molekulargewicht:

Calculated MW: 19.7 kDa

Observed MW: 19 kDa

Gen-ID: 16153

UniProt: P18893

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints