IL36A/IL1F6 Protein (AA 6-158) (His tag)

Produktnummer ABIN7319787

Dieses Recombinant IL36A/IL1F6-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für IL36A/IL1F6 Protein (AA 6-158) (His tag) (ABIN7319787)

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Cell Culture (CC)

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 6-158

Aufreinigungstag / Konjugat: Dieses IL36A/IL1F6 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Interleukin-36 alpha/IL-36 alpha

Sequenz: Lys6-Phe158

Produktmerkmale: Recombinant Human Interleukin-36 Alpha is produced by our E.coli expression system and the target gene encoding Lys6-Phe158 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to induce IL-8 secretion in human PBMCs.The ED₅₀ for this effect is <0.7 ng/mL.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: IL36A/IL1F6 (IL1F6) (Interleukin 1 Family, Member 6 (IL1F6))

Andere Bezeichnung: Interleukin-36 alpha/IL-36 alpha

Hintergrund: FIL,IL1F,Interleukin-1 Family Member,IL-1F,IL36A,FIL1,FIL1(EPSILON),FIL1E,IL-1F6,IL1(EPSILON),IL1F6,FIL1 Epsilon,IL-1 Epsilon,Interleukin-1 Epsilon,Interleukin-1 Family Member 6,Interleukin-36 Alpha,IL1E,IL 1 epsilon,IL 1F6,IL 1H1,IL1RP2,Interleukin 1 epsilon,Interleukin 1 family member 6,Interleukin 1 family member 6 (epsilon),Interleukin 36 alpha,MGC129552,MGC129553,MGC151479,MGC151481,OTTMUSP00000012798,RP23-176J12.4,Human Interleukin-36α (IL-36α) is a secreted cytokine that belongs to the Interleukin 1 cytokine family. IL-36α is expressed in the immune system and the fetal brain, but not in other tissues or multiple hematopoietic cell lines. IL-36α is the only IL-1 family member found to be expressed on T-cells. IL-36α and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-36α inhibits PPARγ expression, which may lead to a reduction in adipocyte differentiation suggesting metabolic effects of this cytokine. IL-36α, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. This suggest that IL-36α may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

Molekulargewicht:

Calculated MW: 18.1 kDa

Observed MW: 17 kDa

Gen-ID: 27179

UniProt: Q9UHA7

Pathways: Cancer Immune Checkpoints