EPH Receptor B4 Protein (EPHB4)

Produktnummer ABIN7195558

Dieses Recombinant EPH Receptor B4-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für EPH Receptor B4 Protein (EPHB4) (ABIN7195558)

Target: EPH Receptor B4 (EPHB4)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human EphB4/HTK Protein

Sequenz: Met 1-Ala 539

Produktmerkmale: The mature form of human EphB4 (NP_004435.3) extracellular domain (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile 100 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: EPH Receptor B4 (EPHB4)

Andere Bezeichnung: EphB4/HTK

Hintergrund: HTK,MYK1,TYRO11,Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Molekulargewicht:

Calculated MW: 57.7 kDa

Observed MW: 65-70 kDa

Gen-ID: 2050

NCBI Accession: NP_004435

UniProt: P54760

Pathways: RTK Signalweg