CNTF Protein

Produktnummer ABIN7319650

Dieses Recombinant CNTF-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CNTF Protein (ABIN7319650)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human CNTF Protein (Active)

Sequenz: Ala2-Met200

Produktmerkmale: Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 25 ng/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS,1 mM Cysteine,PH7.4.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: CNTF

Hintergrund:

Background: Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83 % homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.

Synonym: Ciliary Neurotrophic Factor, CNTF

Molekulargewicht: 22.93 kDa

UniProt: P26441

Pathways: JAK-STAT Signalweg