CD86 Protein (CD86) (His tag)

Produktnummer ABIN7321237

Recombinant CD86-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für CD86 Protein (CD86) (His tag) (ABIN7321237)

Target: CD86

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: HEK-293 Cells

Applikation: ELISA

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses CD86 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Rat CD86/B7-2 Protein (His Tag)

Sequenz: Met 1-Ile 250

Produktmerkmale: A DNA sequence encoding the rat CD86 (NP_064466.1) extracellular domain (Met 1-Ile 250) was expressed, fused with a polyhistidine tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear ranger of 6. 4-160 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: CD86

Andere Bezeichnung: CD86/B7-2

Hintergrund: Activation B7-2 antigen,B7-2,B7.2,B70,CD28LG2,CD86,LAB72,T-lymphocyte activation antigen CD86,CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Molekulargewicht: 26.8 kDa

NCBI Accession: NP_064466

UniProt: A0A9K3Y6P6

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation