CD40 Ligand Protein (CD40LG)

Produktnummer ABIN7319660

Recombinant CD40 Ligand-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CD40 Ligand Protein (CD40LG) (ABIN7319660)

Target: CD40 Ligand (CD40LG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human CD40L/TNFSF5 Protein (E.coli)(Active)

Sequenz: Met113-Leu261

Produktmerkmale: Recombinant Human CD40L is produced by our E.coli expression system and the target gene encoding Met113-Leu261 is expressed.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human CD40L at 5μg/ml(100 μl/well) can bind Human CD40-Fc(Cat: PKSH032216). The ED50 of Human CD40L is 68.55ug/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD40L/TNFSF5

Hintergrund:

Background: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40, its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Synonym: CD40 Ligand, CD40-L, T-Cell Antigen Gp39, TNF-Related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, CD154, CD40LG, CD40L, TNFSF5, TRAP,hCD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP

Molekulargewicht: 16.2 kDa

UniProt: P29965

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints