CD40 Ligand Protein (CD40LG)

Produktnummer ABIN7318281

Dieses Recombinant CD40 Ligand-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für CD40 Ligand Protein (CD40LG) (ABIN7318281)

Target: CD40 Ligand (CD40LG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Bio-Layer Interferometry (BLI)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Verwendungszweck: Recombinant Human CD40L/TNFSF5 Protein (Human Cells)

Sequenz: Met 113-Leu261

Produktmerkmale: Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Loaded Human CD40-Fc on Protein A Biosensor, can bind Human CD40L with an affinity constant of 3.29 nM as determined in BLI assay.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 12 months

Antigendetails

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD40L/TNFSF5

Hintergrund: CD154,CD40 Ligand,CD40-L,CD40L,CD40LG,HIGM1,IGM,IMD3,T-BAM,T-Cell Antigen Gp39,TNF-Related Activation Protein,TNFSF5,TRAP,Tumor Necrosis Factor Ligand Superfamily Member 5,hCD40L,CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40, its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

Molekulargewicht:

Calculated MW: 16.2 kDa

Observed MW: 16-18 kDa

Gen-ID: 959

UniProt: P29965

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints