PD-L1 Protein (His-Avi Tag,Biotin)

Produktnummer ABIN7199143

Dieses Recombinant PD-L1-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für PD-L1 Protein (His-Avi Tag,Biotin) (ABIN7199143)

Target: PD-L1 (CD274 (PD-L1))

Protein-Typ: Recombinant

Spezies: Cynomolgus, Rhesusaffen

Quelle: HEK-293 Cells

Reinheit: >90 % as determined by SDS-PAGE.

Güteklasse: MALS verified

Produktdetails

Aufreinigungstag / Konjugat: Dieses PD-L1 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His,Avitag™ (MALS verified)

Sequenz: Phe 19 - Arg 238

Produktmerkmale: Biotinylated Cynomolgus / Rhesus macaque PD-L1, His,Avitag (PDL-C82E8) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1).

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.8 kDa. The protein migrates as 32-40 kDa under reducing (R) condition due to glycosylation.

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1 / B7-H1

Hintergrund: Synonyms: PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1,
Description: Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Molekulargewicht: 28.8 kDa

Pathways: Cancer Immune Checkpoints