SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag)

Produktnummer ABIN6952653

Dieses Recombinant SARS-CoV-2 Spike S1-Protein wird in HEK-293 Cells exprimiert und wurde in 1 Publikation erwähnt.

Kurzübersicht für SARS-CoV-2 Spike S1 Protein (RBD, V367F) (His tag) (ABIN6952653)

Target: SARS-CoV-2 Spike S1

Protein-Typ: Recombinant

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Applikation: SDS-PAGE (SDS), ELISA

Reinheit: >95 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: RBD, V367F

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His tag.

Verwendungszweck: SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

Sequenz: AA 319-541

Produktmerkmale: SARS-CoV-2 S protein RBD (V367F), His Tag (SPD-S52H4) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (V367F). Predicted N-terminus: Arg 319
This protein carries a polyhistidine tag at the C-terminus.
The V367F mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

Referenzen

Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." in: Nature communications, Vol. 12, Issue 1, pp. 2623, (2021) (PubMed).

Antigendetails

Target: SARS-CoV-2 Spike S1

Substanzklasse: Viral Protein

Hintergrund: It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 27.0 kDa

Gen-ID: 43740568

UniProt: P0DTC2