SARS-CoV-2 Spike S1 Protein (AA 16-685, E484K, K417N, N501Y) (His tag)

Produktnummer ABIN7274391

Dieses Recombinant SARS-CoV-2 Spike S1-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für SARS-CoV-2 Spike S1 Protein (AA 16-685, E484K, K417N, N501Y) (His tag) (ABIN7274391)

Target: SARS-CoV-2 Spike S1

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: SARS Coronavirus-2 (SARS-CoV-2)

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 16-685, E484K, K417N, N501Y

Aufreinigungstag / Konjugat: Dieses SARS-CoV-2 Spike S1 Protein ist gelabelt mit His tag.

Verwendungszweck: SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein

Sequenz: Val16-Arg685(N501Y,K417N,E484K)

Produktmerkmale: Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K), His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.6ng/ml determined by ELISA.,SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS ( pH 7.4).

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: SARS-CoV-2 Spike S1

Substanzklasse: Viral Protein

Hintergrund: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molekulargewicht: 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.

NCBI Accession: YP_009724390